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These results underscore the challenges posed by NACs low oral bioavailability (estimated at 410%) due to extensive first-pass metabolism

Also addition of solution of glutathione (GSH) transferase in the reaction mixture caused a marked increase in the rate of reaction compared to the non-enzymatic, showing monobromobimane was a substrate for this enzyme ( Figure 1 )

Antioxidants 10:1734

In ex vivo conditions, RIPC induces the sequestration of plasma glutamate via EAAT transporters located on the blood cells surface and probably contributes to enhanced glutathione synthesis

There was a risk of a rebound effect if treatment was too short